![]() ![]() It is these antigens that, during an infection, trigger the. For more details, see: Recommendations for Test Performance and Interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease. The results confirmed that elisa is a more reliable screening assay than the IFA test, although false positive and negative serologies were obtained. The Western blot test begins by separating multiple bacterial antigens using gel electrophoresis. ![]() Before CDC will recommend new tests, they must be cleared by the Food and Drug Administration (FDA). New tests may be developed as alternatives to one or both steps of the two-step process. Physicians have commonly ordered the western blot test for Lyme disease even in the absence of positive or equivocal testing. Positive IgM results should be disregarded if the patient has been ill for more than 30 days.ĬDC supports the development of new tests Some tests give results for two types of antibody, IgM and IgG. Antibody-based tests (ELISA, IFA, Western blot, Immunoblot): These tests detect antibodies against Borrelia burgdorferi in the blood or spinal fluid.Infection with other diseases, including some tickborne diseases, or some viral, bacterial, or autoimmune diseases, can result in false positive test results.Antibodies normally persist in the blood for months or even years after the infection is gone therefore, the test cannot be used to determine cure.Antibodies can take several weeks to develop, so patients may test negative if infected only recently.Most Lyme disease tests are designed to detect antibodies made by the body in response to infection.The overall result is positive only when the first test is positive (or equivocal) and the second test is positive (or for some tests equivocal). If the first step is positive or indeterminate (sometimes called “equivocal”), the second step should be performed. If this first step is negative, no further testing is recommended. Both steps are required and can be done using the same blood sample. Results of laboratory tests, when indicatedĬDC currently recommends a two-step testing process for Lyme disease.The possibility that other illnesses may cause similar symptoms.The likelihood that the patient has been exposed to infected blacklegged ticks.medical-conditions/sexual-and-reproductive-health/hiv-aids/diagnosis/western-blot-test.When assessing a patient for Lyme disease, health care providers should consider: Updated CDC recommendation for serologic diagnosis of Lyme disease. ![]() ![]() Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Western blot: Technique, theory, and troubleshooting. aboutAPHL/publications/Documents/ID_HIV-1-1-WesternBlotBrief_62015.pdf
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